Method of determining the heparin content

专利名称：Method of determining the heparin content发明人：Robert Johan Wagenvoord,Hendrik

Coenraad Hemker

申请号：US09/254046申请日：19990301公开号：US06140062A公开日：20001031

摘要：In a method of determining heparin content, a known amount of thrombin (FIIa)or activated coagulation factor X (FXa) is added to a mixture which comprises a knownconcentration of chromogenic substrate (S) , a known concentration of antithrombin (AT)and a sample having an unknown heparin concentration. The amount of FIIa or FXa ischosen such that at most 20%, of the S present is reacted during the period which the ATneeds to deactivate all the FIIa or FXa present. The final concentration of the reactedchromogenic substrate p-nitroanilide ([pNA]. sub.final) is determined after completion ofthe reactions, and the [pNA]. sub.final is used to determine the rate constant (k.sub.dec)of the reaction of FIIa or FXa with AT using the relationship: ##EQU1## The heparinconcentration in the sample can then be determined using the value of k.sub.dec from apredetermined calibration curve of k.sub.dec against heparin concentration.

申请人：UNIVERSITEIT MAASTRICHT

代理机构：Young & Thompson

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