常用的通用引物序列

Primer T7

SP6 T3

pUC/M13 Forward (-40) pUC/M13 Forward (-21) pUC/M13 Reverse T7 Terminator pGEX 5’ pGEX 3’ pQEF pQER polyhedrin F Stag BGH reverse 5’ AOX -factor 3’ AOX

Primer sequence TAATACGACTCACTATAGGG

TATTTAGGTGACACTATAG ATTAACCCTCACTAAAGGGA GTTTTCCCAGTCACGAC TGTAAAACGACGGCCAGT TCACACAGGAAACAGCTATGAC GCTAGTTATTGCTCAGCGG GGGCTGGCAAGCCACGTTTGGTG CCGGGAGCTGCATGTGTCAGAGG GGCGTATCACGAGGCCCTTTCG CATTACTGGATCTATCAACAGG AAATGATAACCATCTCGCAA GAACGCCAGCACATGGACAGC TAGAAGGCACAGTCGAGG GACTGGTTCCAATTGACAAGC TATTGCCAGCATTGCTGC GCAAATGGCATTCTGACATCC

Applicable vectors pGEM-T, pGEM-T-Easy, pCRII, pET, pBlueScript, pcDNA3.1, pT7Blue

pGEM-T, pGEM-T-Easy, pCRII pBlueScript

pUC, pGEM-T, pCRII, pBlueScript pUC, pCRII, pBlueScript

pUC, pGEM-T, pCRII, pBlueScript pET pGEX pGEX pQE pQE pET-4x

pcDNA3.1, pTracer-CMV pPlCZ pPlCZ pPlCZ